diff --git a/pytest.ini b/pytest.ini
index 8345dbf58..3f5eb4b96 100644
--- a/pytest.ini
+++ b/pytest.ini
@@ -132,6 +132,7 @@ flake8-ignore =
     scrapy/utils/iterators.py E501
     scrapy/utils/log.py E128 E501
     scrapy/utils/markup.py F403
+    scrapy/utils/middlewares.py E501
     scrapy/utils/misc.py E501
     scrapy/utils/multipart.py F403
     scrapy/utils/project.py E501