diff --git a/pyproject.toml b/pyproject.toml
index d35b333ff..ea7893e2a 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -126,7 +126,6 @@ ignore_errors = true
 [[tool.mypy.overrides]]
 module = [
     "scrapy.core.downloader.webclient",
-    "scrapy.spiders.init",
     "tests.test_webclient",
 ]
 allow_any_generics = true
@@ -138,7 +137,6 @@ warn_return_any = false
 # usually no type hints
 [[tool.mypy.overrides]]
 module = [
-#    "IPython.*",
     "bpython",
     "brotli",
     "brotlicffi",
diff --git a/scrapy/spiders/init.py b/scrapy/spiders/init.py
deleted file mode 100644
index 957bfffd3..000000000
--- a/scrapy/spiders/init.py
+++ /dev/null
@@ -1,64 +0,0 @@
-from __future__ import annotations
-
-import warnings
-from typing import TYPE_CHECKING, Any, cast
-
-from scrapy.exceptions import ScrapyDeprecationWarning
-from scrapy.spiders import Spider
-from scrapy.utils.spider import iterate_spider_output
-
-if TYPE_CHECKING:
-    from collections.abc import AsyncIterator, Iterable
-
-    from scrapy import Request
-    from scrapy.http import Response
-
-
-class InitSpider(Spider):
-    """Base Spider with initialization facilities
-
-    .. warning:: This class is deprecated. Copy its code into your project if needed.
-    It will be removed in a future Scrapy version.
-    """
-
-    def __init__(self, *args, **kwargs):
-        super().__init__(*args, **kwargs)
-        warnings.warn(
-            "InitSpider is deprecated. Copy its code from Scrapy's source if needed. "
-            "Will be removed in a future version.",
-            ScrapyDeprecationWarning,
-            stacklevel=2,
-        )
-
-    async def start(self) -> AsyncIterator[Any]:
-        with warnings.catch_warnings():
-            warnings.filterwarnings(
-                "ignore", category=ScrapyDeprecationWarning, module=r"^scrapy\.spiders$"
-            )
-            for item_or_request in self.start_requests():
-                yield item_or_request
-
-    def start_requests(self) -> Iterable[Request]:
-        self._postinit_reqs: Iterable[Request] = super().start_requests()
-        return cast("Iterable[Request]", iterate_spider_output(self.init_request()))
-
-    def initialized(self, response: Response | None = None) -> Any:
-        """This method must be set as the callback of your last initialization
-        request. See self.init_request() docstring for more info.
-        """
-        return self.__dict__.pop("_postinit_reqs")
-
-    def init_request(self) -> Any:
-        """This function should return one initialization request, with the
-        self.initialized method as callback. When the self.initialized method
-        is called this spider is considered initialized. If you need to perform
-        several requests for initializing your spider, you can do so by using
-        different callbacks. The only requirement is that the final callback
-        (of the last initialization request) must be self.initialized.
-
-        The default implementation calls self.initialized immediately, and
-        means that no initialization is needed. This method should be
-        overridden only when you need to perform requests to initialize your
-        spider
-        """
-        return self.initialized()
diff --git a/tests/test_spider.py b/tests/test_spider.py
index ff9aed74f..23efed77a 100644
--- a/tests/test_spider.py
+++ b/tests/test_spider.py
@@ -11,10 +11,9 @@ from scrapy.crawler import Crawler
 from scrapy.http import Response, TextResponse, XmlResponse
 from scrapy.settings import Settings
 from scrapy.spiders import CSVFeedSpider, Spider, XMLFeedSpider
-from scrapy.spiders.init import InitSpider
 from scrapy.utils.test import get_crawler, get_reactor_settings
 from tests import get_testdata
-from tests.utils.decorators import coroutine_test, inline_callbacks_test
+from tests.utils.decorators import inline_callbacks_test
 
 
 class TestSpider:
@@ -122,27 +121,6 @@ class TestSpider:
         mock_logger.log.assert_called_once_with("INFO", "test log msg")
 
 
-@pytest.mark.filterwarnings("ignore::scrapy.exceptions.ScrapyDeprecationWarning")
-class TestInitSpider(TestSpider):
-    spider_class = InitSpider
-
-    @coroutine_test
-    async def test_start_urls(self):
-        responses = []
-
-        class TestSpider(self.spider_class):
-            name = "test"
-            start_urls = ["data:,"]
-
-            async def parse(self, response):
-                responses.append(response)
-
-        crawler = get_crawler(TestSpider)
-        await crawler.crawl_async()
-        assert len(responses) == 1
-        assert responses[0].url == "data:,"
-
-
 class TestXMLFeedSpider(TestSpider):
     spider_class = XMLFeedSpider