diff --git a/.bumpversion.cfg b/.bumpversion.cfg
index 53e873427..0bcd1ab01 100644
--- a/.bumpversion.cfg
+++ b/.bumpversion.cfg
@@ -1,5 +1,5 @@
 [bumpversion]
-current_version = 2.10.0
+current_version = 2.10.1
 commit = True
 tag = True
 tag_name = {new_version}
diff --git a/docs/news.rst b/docs/news.rst
index 0154e64ff..9e758f05d 100644
--- a/docs/news.rst
+++ b/docs/news.rst
@@ -20,6 +20,13 @@ Backward-incompatible changes
 
     (:issue:`5968`)
 
+.. _release-2.10.1:
+
+Scrapy 2.10.1 (2023-08-30)
+--------------------------
+
+Marked ``Twisted >= 23.8.0`` as unsupported. (:issue:`6024`, :issue:`6026`)
+
 .. _release-2.10.0:
 
 Scrapy 2.10.0 (2023-08-04)
diff --git a/scrapy/VERSION b/scrapy/VERSION
index 10c2c0c3d..8bbb6e406 100644
--- a/scrapy/VERSION
+++ b/scrapy/VERSION
@@ -1 +1 @@
-2.10.0
+2.10.1
diff --git a/setup.py b/setup.py
index 405633f55..47c0af0b0 100644
--- a/setup.py
+++ b/setup.py
@@ -6,7 +6,8 @@ version = (Path(__file__).parent / "scrapy/VERSION").read_text("ascii").strip()
 
 
 install_requires = [
-    "Twisted>=18.9.0",
+    # 23.8.0 incompatibility: https://github.com/scrapy/scrapy/issues/6024
+    "Twisted>=18.9.0,<23.8.0",
     "cryptography>=36.0.0",
     "cssselect>=0.9.1",
     "itemloaders>=1.0.1",